Breast Cancer Surgery

For patients with early stage breast carcinoma, the ability to achieve complete tumor resection with breast conservation therapy (BCT) remains imperfect despite advances in surgical, pathologic and imaging techniques. The goal of BCT is to remove a patient’s breast cancer with negative surgical margins while achieving a cosmetically acceptable result. The presence or absences of malignant cells on the edge or close to the edge of a lumpectomy specimen is called the surgical margin, and it serves as a marker of residual disease in the breast. 

Currently re-excision rates to achieve clear margins in the United States range between 15-40%. The negative impacts of re-excision include delays in the completion of adjuvant therapies, increased infection rates, increased health care costs, increased mastectomy rates and negative psychological impacts on the patients. The principle of clear margins is imperative since presence of positive margins is associated with increased loco-regional recurrence, and the failure to maintain local control is associated with a decrease in long-term breast cancer specific survival.

In AMIGO, we are studying the use of novel intraoperative mass spectrometry and magnetic resonance imaging (MRI) as methods to detect residual cancer in the hopes of reducing the rates of re-excision for breast cancer patients. AMIGO enables surgeons to incorporate existing and novel imaging technologies including intraoperative MRI and mass spectrometry, to efficiently and precisely guide treatment — before, during, and after surgery — without the patient or medical team ever leaving the operating room.

It is our hypothesis that utilizing intra-procedural mass spectrometry and breast MRI will allow for improved visualization and characterization of tumors and breast-tumor biomarkers that will be effective in assessing tumor margins and areas of residual disease. Ultimately we hope that implementation of these advanced imaging technologies during breast surgery will allow surgeons to achieve more precise excisions and successful first-surgery clear margin rates, ultimately hopefully avoiding the need for further operations.

Mehra Golshan MD FACS
Distinguished Chair in Surgical Oncology

If you are a patient and would like to learn about the offerings of AMIGO, please visit the BWH AMIGO page.

Review the clinical trial here.

Study Contact:
Stephen (Dan) DeSantis 


Breast Conserving Surgery Workflow in AMIGO

Diagnostic Imaging. 3D volume rendered MIP images showing the main mass and satellites. The presence of satellite tumors poses a difficulty in ensuring complete tumor resection.
Diagnostic Imaging. Color overlay by a Computer-aided software over the rapidly enhancing mass (invasive ductal cancer).
initial breast MRI
Preoperative. In the AMIGO operating room the patient is placed under general anesthesia. A contrast enhanced 3D MRI then is performed while the patient is in supine position. In this case, an MRI is performed before and after contrast injection, delineating the tumor boundaries in relationship to fixed landmarks. Upon completion of the preprocedural MRI, the MRI machine leaves the room and the images are evaluated.
breast intraop prep
Intraoperative. The woman's breast is then prepared and draped in standard sterile fashion. Through palpation, the tumor is identified and surgical incisions marked.
breast intraop openingIntraoperative. As part of the lymph node evaluation procedure, marcaine and 2cc of 1% methylene blue diluted with 3cc of saline are injected. The breast is then massaged to distribute the methylene blue to the lymphatics before beginning the incision between the pectorals and latissimus muscles, below the marked line.
breast resectionIntraoperative. The methylene blue renders the sentinel lymph nodes detectable. The lymph nodes sent to pathology for evaluation. After the lumpectomy is performed, the resected tumor is sent to pathology.
breast temp closingIntraoperative. The lumpectomy cavity is temporarily closed with a nylon suture.
breast saline injectionIntraoperative. Before completing closure, the lumpectomy cavity is filled with exact volume of resected tumor and a 1cm margin. Finally, the incision is covered.
Breast PostPostoperative. Immediately post-surgery, a second MRI scan (again, with contrast) is obtained while the patient is on the operating table. The purpose of this MRI is to examine the saline filled cavity to identify areas of enhancement that may be suspicious for residual carcinoma. Following the imaging, the MRI scanner leaves the operating room. A radiologist compares the resected tumor to an MRI of the second breast for possible areas of residual tissue. On the left side, the preoperative images are shown; on the right side, the new images are displayed.
Postoperative. Post-surgical post-contrast MRI showing the saline cavity filled with saline.
Postoperative. 3D volume rendered image showing the surgical cavity.
breast reexision
Re-excision. If suspicious remaining tissue is detected on the MRI images, the breast is reopened and the residual tumor removed.


  1. Mallory MA, Sagara Y, Aydogan F, DeSantis S, Jayender J, Caragacianu D, Gombos E, Vosburgh KG, Jolesz FA, Golshan M. Feasibility of Intraoperative Breast MRI and the Role of Prone Versus Supine Positioning in Surgical Planning for Breast-Conserving Surgery. Breast J. 2017 Mar 10. PubMed PMID: 28295903.
  2. Gombos E.C., Jayender J., Richman D.M., Caragacianu D.L., Mallory M.A., Jolesz F.A., Golshan M.  Intraoperative Supine Breast MR Imaging to Quantify Tumor Deformation and Detection of Residual Breast Cancer: Preliminary Results. Radiology. 2016 Dec;281(3):720-9. PMID: 27332738. PMC5118055.
  3. Gombos EC, Jagadeesan J, Richman DM, Kacher DF. Magnetic Resonance Imaging-Guided Breast Interventions: Role in Biopsy Targeting and Lumpectomies. Magn Reson Imaging Clin N Am. 2015;23 (4) :547-61. Review. PubMed PMID: 26499274;PubMed Central PMCID: PMC4621771.
  4. Jayender J., Chikarmane S., Jolesz F.A., Gombos E. Automatic Segmentation of Invasive Breast Carcinomas from Dynamic Contrast-Enhanced MRi using Time Series Analysis. J Magn Reson Imaging. 2014 Aug;40(2):467-75. PMID: 24115175. PMC3962815.
  5. Golshan M., Sagara Y., Wexelman B., Aydogan F., DeSantis S., Elise Min H., Vosburgh K., Jagadeesan J., Caragacianu D., Gombos E., Jolesz F.A. Pilot Study to Evaluate Feasibility of Image-Guided Breast-Conserving Therapy in the Advanced Multimodal Image-Guided Operating (AMIGO) suite. Ann Surg Oncol. 2014 Oct;21(10):3356-7. PMID: 250474. PMC4697273.
  6. Calligaris D., Caragacianu D., Liu X., Norton I., Thompson C.J., Richardson A.L., Golshan M., Easterling M.L., Santagata S., Dillon D.A., Jolesz F.A., Agar N.Y.R. Application of Desorption Electrospray Ionization Mass Spectrometry Imaging in Breast Cancer Margin Analysis. Proc Natl Acad Sci U S A. 2014;111 (42) :15184-9. PMID: 25246570. PMC4210338.

Book Chapters

  1. Eva C. Gombos, Daniel F. Kacher, Diana L. Caragacianu, Jagadeesan Jayender, Mehra Golshan. Magnetic Resonance Imaging-Guided Breast Intervention and Surgery. Ch.62. Part V. Image-Guided Clinical Applications. In Ferenc A. Jolesz (Ed.), Intraoperative Imaging and Image-Guided Therapy. New York, NY: Springer; 2014. pp. 821-48.

Media Coverage

  1. Amigo Innovative Breast Cancer Surgery. Nexstar Broadcasting, Inc.- August 14, 2013
  2. New Operating Room at BWH Helps Breast Cancer Patients Avoid Multiple Surgeries. CBS Boston - December 7, 2012
  3. Reducing Repeat Surgeries After Breast Cancer. BWH Health Hub – January, 2013
  4. The Fine Line Between Breast Cancer and Normal Tissue. MedicalPress – September, 2014